hTNF alpha

Regulatory Status: RUO

Other Names: Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF).

Description: Differentiation inducing factor (DIF), TNFSF2. TNF-α is released from macrophages, monocytes, neutrophils, T-cells (principally CD4+), NK-cells and many transformed cell lines. Soluble homotrimeric TNF-α is released from cells by proteolysis of the integral membrane precursor form of TNF-α. TNF-α binding to some TNF-α receptors induces apoptosis and depending on cell type, receptor expression, and signal transduction status can induce other responses. TNF-α is involved in the inflammatory response.

PRODUCT DETAILS

Source: Human TNF-α, amino acids Val77-Leu233 (Accession# NM 000594 UNIPROT), was expressed in E. coli.

Molecular Mass: The 157 amino acid recombinant protein has a predicted molecular mass of 17,352 Da. The DTT-reduced protein and non-reduced protein migrate at approximately 16kDa by SDS-PAGE. The N-terminal amino acid is Val.

Purity: Purity is >98%, as determined by Coomassie stained SDS-PAGE.

Formulation: 0.22 µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH 7.2.

Concentration: 10 and 50 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration

Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Application: Bioassay