hIFN gamma

Regulatory Status: RUO

Other Names: Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF).

Description: Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

PRODUCT DETAILS

Source: Human IFN-γ, amino acids Gln24-Gln166 (Accession # NM P01579 UNIPROT) was expressed in E. coli.

Molecular Mass: The 144 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16,907 Da. The DTT-reduced protein and the non-reduced protein migrate at approximately 17 kDa by SDS-PAGE.

Purity: Purity is >95%, as determined by Coomassie stained SDS-PAGE.

Formulation: 0.22 µm filtered protein solution is in 10mM Sodium succinate, 4% Mannitol, pH 5.0.

Concentration: 10 and 25 µg sizes are bottled at 100 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, 

Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Application: Bioassay