{"product_id":"recombinant-human-epidermal-growth-factor-rhegf","title":"Recombinant Human Epidermal Growth Factor (rhEGF)","description":"\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eProduct Information\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePart Number:\u003c\/span\u003e\u003cspan\u003e GF001\u003c\/span\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003cspan\u003eProduct Name:\u003c\/span\u003e\u003cspan\u003e Epidermal Growth Factor\u003c\/span\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003cspan\u003eSynonyms:\u003c\/span\u003e\u003cspan\u003e EGF, Epidermal Growth Factor, Urogastrone, URG\u003c\/span\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003cspan\u003eQuantity:\u003c\/span\u003e\u003cspan\u003e 200 µg\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eEpidermal growth factor (EGF) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. EGF signals through the EGF receptor (EGFR\/ErbB1), which can heterodimerize with ErbB2, ErbB3, or ErbB4, and may also associate with PDGF receptor beta and HGF receptor (c-MET). During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult organism, EGF plays roles in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eProduced in \u003c\/span\u003e\u003cspan\u003ePichia pastoris\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThe recombinant protein corresponds to the active domain of human EGF (aa 971–1023) fused at its C-terminus to six additional arginine residues.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eMolecular Characteristics\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eProtein length:\u003c\/span\u003e\u003cspan\u003e 59 amino acids\u003c\/span\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003cspan\u003eCalculated molecular weight:\u003c\/span\u003e\u003cspan\u003e 7,159.14 Da\u003c\/span\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003cspan\u003eIsoelectric point (pI):\u003c\/span\u003e\u003cspan\u003e 8.30\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eGenBank Accession:\u003c\/span\u003e\u003cspan\u003e AAH93731.1\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eProduct Specifications\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eConcentration\u003c\/span\u003e\u003cspan\u003e: 1.0 mg\/mL after reconstitution in 100 µL sterile water\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eFormulation:\u003c\/span\u003e\u003cspan\u003e Lyophilized from sterile PBS, pH 7.4\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePurity: \u003c\/span\u003e\u003cspan\u003e≥97% (SDS-PAGE)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eEndotoxin Level\u003c\/span\u003e\u003cspan\u003e: \u0026lt;0.1 EU\/µg protein (Pyrosate® Kit; Associates of Cape Cod)\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eReconstitution\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eReconstitute the lyophilized protein with 100 µL sterile water.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eFurther dilutions should be prepared using culture medium containing 5% fetal bovine serum (FBS).\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eShipped at ambient temperature.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eStore at 2–8 °C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eFor long-term storage, keep at −20 °C.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eHandling\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eAvoid repeated freeze–thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eAfter reconstitution, prepare aliquots and store at −20 °C.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eQuality Control\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e97% as determined by SEC-HPLC.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eBiological Activity\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThe biological activity of human EGF is defined by its Median Effective Dose (ED50), determined through a dose-dependent mitogenic activity assay using BALB\/c 3T3 cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eHistorical batches have shown an ED50 activity ≤ 0.1 ng\/mL.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eAmino Acid Sequence (59 aa)\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRRRRRRR\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Comercializadora Centro de Biotecnología y Biomedicina Ltda.","offers":[{"title":"Default Title","offer_id":43452842377334,"sku":null,"price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0578\/2532\/9270\/files\/unnamed_12.png?v=1780068720","url":"https:\/\/bertosbiotech.com\/products\/recombinant-human-epidermal-growth-factor-rhegf","provider":"Bertosbiotech","version":"1.0","type":"link"}