{"product_id":"recombinant-human-brain-derived-neurotrophic-factor-rhbdnf","title":"Recombinant Human Brain-Derived Neurotrophic Factor (rhBDNF)","description":"\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eProduct Information\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePart Number:\u003c\/span\u003e\u003cspan\u003e GF002\u003c\/span\u003e\u003cspan\u003e\u003cspan class=\"Apple-tab-span\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eProduct Name:\u003c\/span\u003e\u003cspan\u003e Brain-Derived Neurotrophic Factor\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eSynonyms:\u003c\/span\u003e\u003cspan\u003e BDNF, Abrineurin\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eQuantity:\u003c\/span\u003e\u003cspan\u003e 100 µg\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eBrain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors, which also includes nerve growth factor (NGF), neurotrophin-3 (NT-3), and neurotrophin-4\/5 (NT-4\/5). BDNF plays an essential role in the survival, differentiation, and maintenance of neuronal populations in both the central and peripheral nervous systems. It is widely expressed in the brain, particularly in the hippocampus, cortex, and cerebellum.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eBDNF promotes neuronal survival, neurite outgrowth, synaptic plasticity, and neuronal differentiation. Its biological effects are mediated primarily through activation of the TrkB receptor tyrosine kinase, which initiates intracellular signaling pathways involved in neuronal growth and survival.Alterations in BDNF expression or signaling have been associated with several neurological disorders, including neurodegenerative diseases and mood disorders.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eRecombinant protein produced in Escherichia coli (E. coli).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThe recombinant protein contains a C-terminal spacer peptide (4 aa) followed by a six\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ehistidine tag (6×His).\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eMolecular Characteristics\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eProtein length:\u003c\/span\u003e\u003cspan\u003e 131 amino acids\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eCalculated molecular weight:\u003c\/span\u003e\u003cspan\u003e 14,807 Da\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eSDS-PAGE migration:\u003c\/span\u003e\u003cspan\u003e Approximately 15 kDa under both reducing (DTT) and non-reducing conditions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eProduct Specifications\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eConcentration\u003c\/span\u003e\u003cspan\u003e: 1 mg\/mL after reconstitution in 100 µL sterile water\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePurity: \u003c\/span\u003e\u003cspan\u003e≥97% (SDS-PAGE)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eEndotoxin Level\u003c\/span\u003e\u003cspan\u003e: \u0026lt;0.1 EU\/µg protein (Pyrosate® Kit; Associates of Cape Cod)\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eReconstitution\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eReconstitute the lyophilized protein with 100 µL sterile water.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eFurther dilutions should be prepared using medium containing 5% fetal bovine serum (FBS).\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eShipped at ambient temperature.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eStore at 2–8 °C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eFor long-term storage, keep at −20 °C.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eHandling\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eAvoid repeated freeze–thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eAfter reconstitution, prepare aliquots and store at −20 °C.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eQuality Control\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eBiological Activity\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThe biological activity of recombinant human BDNF can be determined using cell-based assays that measure TrkB receptor activation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eCommon bioassays include:\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli dir=\"ltr\" aria-level=\"1\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eCell proliferation assays using BaF3 cells engineered to express TrkB\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\" aria-level=\"1\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eNeuronal survival assays\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\" aria-level=\"1\"\u003e\n\u003cp dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eNeurite outgrowth assays in primary neuronal cultures\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThese assays evaluate the ability of BDNF to activate TrkB-mediated signaling pathways that promote neuronal survival and differentiation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003cbr\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eAmino Acid Sequence (131 aa)\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eMHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGRNGGGGHHHHHH\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"CBB Animal Health","offers":[{"title":"Default Title","offer_id":43452289253494,"sku":null,"price":450.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0578\/2532\/9270\/files\/unnamed_13.png?v=1780069297","url":"https:\/\/bertosbiotech.com\/products\/recombinant-human-brain-derived-neurotrophic-factor-rhbdnf","provider":"CBB Animal Health","version":"1.0","type":"link"}