{"product_id":"recombinant-canine-vascular-endothelial-growth-factor-120-rcvegf120","title":"Recombinant Canine Vascular Endothelial Growth Factor 120 (rcVEGF120)","description":"\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eProduct Information\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePart Number:\u003c\/span\u003e\u003cspan\u003e GF002\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eProduct Name:\u003c\/span\u003e\u003cspan\u003e Canine Vascular Endothelial Growth Factor 120\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eSynonyms:\u003c\/span\u003e\u003cspan\u003e VEGF-A, Vascular Endothelial Growth Factor A, VEGF120\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eQuantity:\u003c\/span\u003e\u003cspan\u003e 100 µg\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eVascular endothelial growth factor (VEGF) is a key regulator of angiogenesis and vasculogenesis. It belongs to a family of secreted growth factors that stimulate the proliferation, migration, and survival of endothelial cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eVEGF exerts its biological activity through binding to VEGF receptor tyrosine kinases (VEGFR-1\/Flt-1 and VEGFR-2\/KDR) located on endothelial cells. Activation of these receptors triggers intracellular signaling pathways including MAPK, PI3K\/AKT, and PLCγ, leading to endothelial cell proliferation, vascular permeability, and formation of new blood vessels.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eVEGF is essential for vascular development, wound healing, and tissue regeneration, and dysregulated VEGF signaling is implicated in tumor angiogenesis and inflammatory diseases.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eMultiple isoforms of VEGF exist due to alternative splicing, including VEGF121, VEGF165, and VEGF189 in humans. The canine VEGF120 isoform represents the shorter soluble form lacking heparin-binding domains, resulting in increased diffusibility in the extracellular environment.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eProduced in Escherichia coli (E. coli).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThe recombinant canine VEGF120 protein contains a C-terminal spacer peptide (2 aa) followed by a six-histidine tag (6×His) to facilitate purification.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eMolecular Characteristics\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eProtein length:\u003c\/span\u003e\u003cspan\u003e 129 amino acids\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eCalculated molecular weight:\u003c\/span\u003e\u003cspan\u003e 15,117 Da. VEGF proteins naturally form disulfide-linked homodimers. After refolding, the recombinant protein is predominantly present in the dimeric form, although SDS-PAGE analysis may reveal both monomeric (~15 kDa) and dimeric (~30 kDa) species even under reducing conditions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eProduct Specifications\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eQuantity:\u003c\/span\u003e\u003cspan\u003e 100 µg\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eFormulation:\u003c\/span\u003e\u003cspan\u003e Lyophilized from \u003c\/span\u003e\u003cspan\u003ePBS\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePurity:\u003c\/span\u003e\u003cspan\u003e ≥96% (SDS-PAGE)\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eReconstitution\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eReconstitute the lyophilized protein with sterile water.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eFurther dilutions should be prepared using medium containing 5% fetal bovine serum (FBS).\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eShipped at ambient temperature.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eStore at 2–8 °C. For long-term storage, keep at −20 °C.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eHandling\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eAvoid repeated freeze–thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eAfter reconstitution, prepare aliquots and store at −20 °C.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eQuality Control\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e≥96% as determined by SDS-PAGE under reducing conditions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eBiological Activity\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThe biological activity of recombinant VEGF is determined using a cell proliferation assay with endothelial cells, typically human umbilical vein endothelial cells (HUVEC). In this assay, VEGF stimulates proliferation of endothelial cells in a dose-dependent manner, and activity is quantified by measuring cell growth relative to untreated controls. This endothelial cell proliferation assay is widely used as a standard functional test for VEGF bioactivity, reflecting activation of VEGF receptor signaling pathways.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eAmino Acid Sequence (129 aa)\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eMAPMAGGEHKPHEVVKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEEFNITMQIMRIKPHQGQHIGEMSFLQHSKCECRPKKDRARQEKCDKPRRGGHHHHHH\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Comercializadora Centro de Biotecnología y Biomedicina Ltda.","offers":[{"title":"Default Title","offer_id":43452843065462,"sku":null,"price":350.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0578\/2532\/9270\/files\/unnamed_14.png?v=1780069929","url":"https:\/\/bertosbiotech.com\/products\/recombinant-canine-vascular-endothelial-growth-factor-120-rcvegf120","provider":"Bertosbiotech","version":"1.0","type":"link"}