{"product_id":"recombinant-human-interleukin-4-rhil-4","title":"Recombinant Human Interleukin-4 (rhIL-4)","description":"\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eProduct Information\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePart Number:\u003c\/span\u003e\u003cspan\u003e C004\u003c\/span\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003cspan\u003eProduct Name:\u003c\/span\u003e\u003cspan\u003e Interleukin-4\u003c\/span\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003cspan\u003eSynonyms:\u003c\/span\u003e\u003cspan\u003e Interleukin-4, BCGF-1, BCGF1, BSF-1, BSF1, IL-4\u003c\/span\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003cspan\u003eQuantity:\u003c\/span\u003e\u003cspan\u003e 100 µg\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eInterleukin-4 (IL-4) is a pleiotropic cytokine that regulates various T-cell and B-cell responses, including the differentiation of naïve T cells into the TH2 phenotype, promoting B-cell proliferation, antibody isotype switching, and the expression of other TH2 cytokines, including IL-5 and IL-9. IL-4 plays a key role in the development of allergic inflammation and asthma.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eProduced in Escherichia coli (E. coli). The recombinant protein contains a C-terminal spacer peptide (4 aa) and a six-histidine tag (6×His).\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eMolecular Characteristics\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eProtein length:\u003c\/span\u003e\u003cspan\u003e 140 amino acids\u003c\/span\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003cspan\u003eCalculated molecular weight:\u003c\/span\u003e\u003cspan\u003e 16,145 Da\u003c\/span\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003cspan\u003eSDS-PAGE migration:\u003c\/span\u003e\u003cspan\u003e Approximately 16 kDa under both reducing (DTT) and non-reducing conditions\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eGenBank Accession:\u003c\/span\u003e\u003cspan\u003e AAA59149.1\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eProduct Specifications\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eConcentration\u003c\/span\u003e\u003cspan\u003e: 1 mg\/mL after reconstitution in 100 µL sterile water\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eFormulation\u003c\/span\u003e\u003cspan\u003e: Lyophilized from 50 mM phosphate buffer, pH 7.4\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003cspan\u003e: ≥90% (SDS-PAGE)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eEndotoxin Level\u003c\/span\u003e\u003cspan\u003e: \u0026lt;0.1 EU\/µg protein (Pyrosate® Kit; Associates of Cape Cod)\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eReconstitution\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eReconstitute the lyophilized protein with 100 µL sterile water.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eFurther dilutions should be prepared using medium containing 5% fetal bovine serum (FBS).\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eShipped at ambient temperature.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eStore at 2–8 °C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eFor long-term storage, keep at −20 °C.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eHandling\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eAvoid repeated freeze–thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eAfter reconstitution, prepare aliquots and store at −20 °C.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eQuality Control\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e90% as determined by SDS-PAGE under reducing conditions and visualized by Coomassie Blue staining.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eBiological Activity\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThe IC50 value (i.e., the concentration of cytotoxic agent required to reduce cell growth by 50%) for rhIL-4 is determined in a bioassay using human TF-1 cells. Historical lots have shown an IC50 activity in the concentration range of: 1–5 ng\/mL\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eAmino Acid Sequence (140 aa)\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eMHKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSSGGGGHHHHHH\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Comercializadora Centro de Biotecnología y Biomedicina Ltda.","offers":[{"title":"Default Title","offer_id":43453119856758,"sku":null,"price":650.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0578\/2532\/9270\/files\/unnamed_16.png?v=1780071787","url":"https:\/\/bertosbiotech.com\/es\/products\/recombinant-human-interleukin-4-rhil-4","provider":"Bertosbiotech","version":"1.0","type":"link"}