{"product_id":"recombinant-bovine-prolactin-rbprl","title":"Recombinant Bovine Prolactin (rbPRL)","description":"\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eProduct Information\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePart Number:\u003c\/span\u003e\u003cspan\u003e H001\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eProduct Name:\u003c\/span\u003e\u003cspan\u003e Bovine Prolactin\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eSynonyms:\u003c\/span\u003e\u003cspan\u003e PRL, Prolactin, Lactotropin, Luteotropic Hormone\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eQuantity:\u003c\/span\u003e\u003cspan\u003e 50 µg\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eProlactin (PRL) is a pituitary peptide hormone of the somatotropin\/prolactin family that plays a central role in the regulation of mammary gland development and lactation. In addition to its classical lactogenic function, prolactin is involved in reproduction, growth, development, and osmoregulatory and immunomodulatory processes. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eProlactin exerts its biological effects through binding to the prolactin receptor (PRLR), a class I cytokine receptor, leading to activation of intracellular signaling pathways that include JAK2\/STAT5 and that regulate proliferation, differentiation, and hormone-responsive gene expression. \u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eProduced in Escherichia coli (E. coli).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThe recombinant protein contains a C-terminal spacer peptide (4 aa) followed by a six-histidine tag (6×His) to facilitate purification.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eMolecular Characteristics\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eProtein length:\u003c\/span\u003e\u003cspan\u003e 243 amino acids\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eCalculated molecular weight:\u003c\/span\u003e\u003cspan\u003e 27,332 Da\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eProduct Specifications\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eQuantity:\u003c\/span\u003e\u003cspan\u003e 100 µg\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eFormulation:\u003c\/span\u003e\u003cspan\u003e Lyophilized from \u003c\/span\u003e\u003cspan\u003ePBS\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePurity:\u003c\/span\u003e\u003cspan\u003e ≥93% (SDS-PAGE)\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eReconstitution\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eReconstitute the lyophilized protein with sterile water.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eFurther dilutions should be prepared using medium containing 5% fetal bovine serum (FBS).\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eShipped at ambient temperature.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eStore at 2–8 °C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eFor long-term storage, keep at −20 °C.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eHandling\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eAvoid repeated freeze–thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eAfter reconstitution, prepare aliquots and store at −20 °C.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eQuality Control\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e≥93% as determined by SDS-PAGE under reducing conditions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eBiological Activity\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThe biological activity of recombinant bovine prolactin is determined using a cell proliferation assay with Nb2-11 rat lymphoma cells. In this assay, prolactin stimulates proliferation of Nb2-11 cells in a dose-dependent manner, and activity is quantified by measuring cell growth relative to untreated controls. Nb2\/Nb2-11 cells are a well-established bioassay system for lactogenic hormones such as prolactin, and this assay is widely used in the literature and by cell repositories for prolactin bioactivity testing. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eSpecific activity \/ ED50: To avoid overstatement, this should be determined experimentally for the final recombinant bovine construct and lot. Commercial recombinant prolactin products from other species often report Nb2-11-based ED50 values, but those values should not be assigned directly to this bovine construct without in-house testing. \u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eAmino Acid Sequence (243 aa)\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eMKETAAAKFERQHMDSPDLGTLVPRGSMADIGSTPVCPNGPGNCQVSLRDLFDRAVMVSHYIHDLSSEMFNEFDKRYAQGKGFITMALNSCHTSSLPTPEDKEQAQQTHHEVLMSLILGLLRSWNDPLYHLVTEVRGMKGAPDAILSRAIEIEEENKRLLEGMEMIFGQVIPGAKETEPYPVWSGLPSLQTKDEDARYSAFYNLLHCLRRDSSKIDTYLKLLNCRIIYNNNCSGGGGHHHHHH\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Comercializadora Centro de Biotecnología y Biomedicina Ltda.","offers":[{"title":"Default Title","offer_id":43452782968950,"sku":null,"price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0578\/2532\/9270\/files\/unnamed_11.png?v=1780068371","url":"https:\/\/bertosbiotech.com\/es\/products\/recombinant-bovine-prolactin-rbprl","provider":"Bertosbiotech","version":"1.0","type":"link"}